Bio-Trac: RNA-Seq – Principles, Methods & Computational Analysis

5 Day Training Workshop

This 5-Day workshop combines a unique mix of hands-on wet bench and electronic laboratory experiences together with a series of lectures spanning an introduction to the concepts of RNA sequencing and the analysis of the data generated to the applications of these approaches to understand important biological and/or biomedical processes and problems. Attendees will have had hands-on experience with RNA sample preparation and differences in sample integrity, a deep understanding of RNA library creation and optimization, upstream processing of RNA sequences, and the hands-on application of the most current programs to analyze developing mouse brain and human knockout cell lines.

Overview of lecture topics:

• Introduction to Sequencing and Course Overview
• Review of NGS and Illumina Technologies
• Library Prep and quantification
• RNA Library Preparation and Quantification
• Introduction to QC, Trimming, alignment, and quantification
• Introduction to Differential Expression and Splicing Tools
• Experiment Zoo: Experience across over 100 RNAseq experiments
• Introduction to variant calling, Enrichment analysis
• Spatially-resolved Transcriptomics
• Advanced Topics in RNA-Seq